NAD+

Pyridine dinucleotide coenzyme. NAD+ (nicotinamide adenine dinucleotide, oxidised form) is a coenzyme: two nucleotides, one carrying nicotinamide and the other adenine, joined through a pair of bridging phosphate groups. It is the one non-peptide material in the Brightly catalog, and it is characterised accordingly, by molecular structure rather than by amino-acid sequence. Brightly supplies it as a lyophilized (freeze-dried) powder in a sealed, tamper-evident vial, prepared and handled strictly as a characterised reference material for in-vitro laboratory research.

NAD+ is one half of a redox pair, and which half you have is the defining property of the material as supplied:

Because each vial contains a single, defined compound at a verified purity rather than a mixture of redox states, NAD+ gives researchers a consistent reference point for redox-biology and enzyme-cofactor work, where an uncertain NAD+/NADH ratio in the test article would confound the measurement being made.

Supplied strictly as a characterised reference material for in-vitro and preclinical laboratory work, not for any diagnostic or therapeutic purpose.

Research overview

NAD+ is supplied as a characterised reference material for controlled in-vitro and preclinical laboratory work. It is manufactured and purified to industry-standard protocols, making it suitable for analytical testing, enzyme-cofactor assays, and experimental bioenergetics models. Every lot is verified by HPLC for purity and identity, with a lot-specific Certificate of Analysis included.

Research-grade standards behind each batch

• Manufactured by controlled enzymatic and chemical synthesis
• Purified using preparative chromatography
• Verified through analytical quality-control procedures
• Screened for endotoxins by LAL
• Identity confirmed by HPLC

Why researchers study NAD+

Published biochemical literature describes NAD+ as a central redox cofactor and as a consumed substrate for several enzyme families,¹ which makes it of interest both as an assay reagent and as a subject of study in its own right. Areas of scientific interest include:

• Redox biology and the NAD+/NADH ratio
• Mitochondrial bioenergetics
• Sirtuin-dependent deacetylation
• PARP-mediated DNA-repair pathways
• CD38 and NAD-glycohydrolase activity
• Cellular-senescence and ageing-biology models

Two distinct roles in the cell

NAD+ is studied along two separate lines, and the distinction matters when designing an experiment around it.

As a redox cofactor. NAD+ accepts a hydride to become NADH and is regenerated on the return leg, so it cycles rather than being consumed. This is the role it plays in glycolysis, the citric-acid cycle, and oxidative phosphorylation.

As a consumed substrate. Sirtuins, PARPs, and CD38 cleave NAD+ rather than cycling it, releasing nicotinamide. Because these enzymes deplete the pool, NAD+ availability is studied as a variable in its own right rather than as a fixed background quantity.

Coenzyme vs peptide reference materials

Reference. Covarrubias AJ, Perrone R, Grozio A, Verdin E. NAD+ metabolism and its roles in cellular processes during ageing. Nat Rev Mol Cell Biol. 2021;22(2):119–141.

Supplied strictly as a characterised reference material for in-vitro and preclinical laboratory work, not for any diagnostic or therapeutic purpose.

Every lot of NAD+ follows a controlled, pharma-grade workflow from synthesis to fulfilment, so the material stays consistent, pure, and stable.

Cleanroom manufacturing

Produced in controlled, low-contamination environments for batch-to-batch consistency.

≥99% HPLC-verified purity

Purity confirmed by high-performance liquid chromatography (HPLC).

Verified identity & purity

Identity and purity confirmed by HPLC, and endotoxin by Limulus Amebocyte Lysate (LAL).

Endotoxin-controlled

Low endotoxin levels matter in sensitive cell-based and enzyme assays, where inflammatory contaminants could confound results.

Pharma-grade raw materials

Synthesis begins with tested precursors and validated reagents to protect molecular integrity.

Lyophilized for stability

Freeze-drying preserves the compound during shipping, storage, and handling; NAD+ is hygroscopic and degrades in solution, so it is supplied dry.

Nitrogen-sealed vials

Vials are sealed under nitrogen to limit exposure to oxygen and moisture.

Understanding lyophilization. Freeze-drying removes moisture by freezing the material and lowering pressure so water sublimates directly from solid to vapour, leaving a stable, dry powder that tolerates storage well for long-term studies.

Before reconstitution. Unopened lyophilized NAD+ tolerates short periods at room temperature in a cool, dry place away from heat, humidity, and light. For longer storage, keep sealed vials frozen: −20 °C is common, while −80 °C adds margin for long-term or archival storage.

After reconstitution. Reconstitute with bacteriostatic water, then keep the solution refrigerated below 4 °C, aliquot to working volumes, and avoid repeated freeze-thaw cycles. Under appropriate laboratory conditions, reconstituted solutions typically remain stable for up to 30 days, following your own laboratory protocol.

Same-day dispatch (USA)

In-stock orders placed before 1:00 PM CT ship the same business day.

Fast domestic delivery

Free FedEx shipping on every order; express delivery is free on orders over $150.

Protective handling

Vials are held in temperature-controlled storage before shipment; the lyophilized powder is stable for ambient transit and is packed to limit temperature swings, moisture, and physical damage.

Tracking and guarantee

Tracking is provided at shipment, and every order is backed by our 30-day money-back guarantee.

See our Shipping and Refund policies for full details.